| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ritchie, Matthew J (9)
|
UNAT |
30.81 |
31.31 |
0.50 |
| 2 |
Delos Santos, Joshua B (8)
|
NCGFSC |
32.27 |
31.68 |
-0.59 |
| 3 |
Stewardson, Bridger D (10)
|
NCVACB |
32.44 |
32.44 |
0.00 |
| 4 |
Garrett, Logan F (10)
|
NCYBAC |
34.09 |
33.56 |
-0.53 |
| 5 |
Clayton, Thomas J (9)
|
NCGFSC |
33.88 |
34.29 |
0.41 |
| 6 |
Rabon, Matthew R (10)
|
NCGFSC |
36.28 |
35.25 |
-1.03 |
| 7 |
Jones, Noah C (9)
|
NCYBAC |
38.64 |
35.31 |
-3.33 |
| 8 |
Earl, Tyler K (10)
|
UNAT |
34.29 |
36.30 |
2.01 |
| 9 |
Pendleton, Zackary A (10)
|
NCGFSC |
37.97 |
37.96 |
-0.01 |
| 10 |
Johnson, Connor R (10)
|
NCGOLD |
40.08 |
38.94 |
-1.14 |
| 11 |
Bacon, Ty M (10)
|
NCGFSC |
39.62 |
40.27 |
0.65 |
| 12 |
Rodeffer, Matthew J (9)
|
NCLTNC |
|
42.37 |
- |
| 13 |
Wozniak, Dominic I (9)
|
NCRMY |
|
42.64 |
- |
| 14 |
Price, Ethan R (10)
|
NCRMY |
43.54 |
44.20 |
0.66 |
| 15 |
Upadhyay, Apurva N (10)
|
NCGFSC |
44.98 |
44.22 |
-0.76 |
| 16 |
Parker, Wyatt T (10)
|
NCCPDD |
|
53.93 |
- |
| 17 |
Dettman, Jonah H (9)
|
NCGFSC |
|
1:00.88 |
- |
|
Honeycutt, Joseph C (8)
|
NCVACB |
|
NS |
- |